PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G04150.1
Common NameBHLH101, EN10, F21E1.70
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 240aa    MW: 27716.5 Da    PI: 10.3527
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G04150.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH41.32.7e-1366115152
                  CHHHHHHHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHH CS
          HLH   1 rrrahnerErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIk 52 
                  ++ +hn+ Er RR+++N  ++ Lr llP  +   +++Kls  +++ ++v+YI 
  AT5G04150.1  66 KKLNHNASERDRRRKLNALYSSLRALLPLsD---QKRKLSIPMTVARVVKYIP 115
                  6789*************************66...9999**************6 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474597.72E-1365136IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000832.23E-1065122No hitNo description
PROSITE profilePS5088814.05665117IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.105.7E-1066122IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.5E-1066115IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003532.4E-871123IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006357Biological Processregulation of transcription from RNA polymerase II promoter
GO:0010106Biological Processcellular response to iron ion starvation
GO:0055072Biological Processiron ion homeostasis
GO:0090575Cellular ComponentRNA polymerase II transcription factor complex
GO:0000977Molecular FunctionRNA polymerase II regulatory region sequence-specific DNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0009005anatomyroot
PO:0009025anatomyvascular leaf
PO:0007115developmental stageLP.04 four leaves visible stage
Sequence ? help Back to Top
Protein Sequence    Length: 240 aa     Download sequence    Send to blast
MEYPWLQSQV HSFSPTLHFP SFLHPLDDSK SHNINLHHMS LSHSNNTNSN NNNYQEEDRG  60
AVVLEKKLNH NASERDRRRK LNALYSSLRA LLPLSDQKRK LSIPMTVARV VKYIPEQKQE  120
LQRLSRRKEE LLKRISRKTH QEQLRNKAMM DSIDSSSSQR IAANWLTDTE IAVQIATSKW  180
TSVSDMLLRL EENGLNVISV SSSVSSTARI FYTLHLQMRG DCKVRLEELI NGMLLGLRQS
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible245692_at0.0
Expression AtlasAT5G04150-
AtGenExpressAT5G04150-
ATTED-IIAT5G04150-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Flowers. {ECO:0000269|PubMed:12679534}.
Function -- GeneRIF ? help Back to Top
  1. up-regulated by iron deficiency in roots and leaves
    [PMID: 17516080]
  2. bHLH100 and bHLH101 are key regulators of iron-deficiency responses independent of the master regulator FIT
    [PMID: 22984573]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G04150.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up regulated by iron deficiency in roots and leaves, as well as by nickel, high zinc or high copper treatments. Repressed by high iron, low copper and low zinc treatments. {ECO:0000269|PubMed:17516080}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Interaction ? help Back to Top
Source Intact With
BioGRIDAT5G11260
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G04150
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ5198100.0AJ519810.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_196035.11e-176transcription factor bHLH101
SwissprotQ9FYE61e-178BH101_ARATH; Transcription factor bHLH101
TrEMBLD7LXG01e-137D7LXG0_ARALL; Predicted protein
STRINGAT5G04150.11e-176(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM48228153
Representative plantOGRP28261129
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  3. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  4. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  5. Underwood BA,Vanderhaeghen R,Whitford R,Town CD,Hilson P
    Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations.
    Plant Biotechnol. J., 2006. 4(3): p. 317-24
    [PMID:17147637]
  6. Vorwieger A, et al.
    Iron assimilation and transcription factor controlled synthesis of riboflavin in plants.
    Planta, 2007. 226(1): p. 147-58
    [PMID:17260143]
  7. Wang HY, et al.
    Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana.
    Planta, 2007. 226(4): p. 897-908
    [PMID:17516080]
  8. Gong W, et al.
    The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors.
    Mol Plant, 2008. 1(1): p. 27-41
    [PMID:19802365]
  9. Golisz A,Sugano M,Hiradate S,Fujii Y
    Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA.
    Planta, 2011. 233(2): p. 231-40
    [PMID:20978802]
  10. Ivanov R,Brumbarova T,Bauer P
    Fitting into the harsh reality: regulation of iron-deficiency responses in dicotyledonous plants.
    Mol Plant, 2012. 5(1): p. 27-42
    [PMID:21873619]
  11. Wang N, et al.
    Requirement and functional redundancy of Ib subgroup bHLH proteins for iron deficiency responses and uptake in Arabidopsis thaliana.
    Mol Plant, 2013. 6(2): p. 503-13
    [PMID:22983953]
  12. Sivitz AB,Hermand V,Curie C,Vert G
    Arabidopsis bHLH100 and bHLH101 control iron homeostasis via a FIT-independent pathway.
    PLoS ONE, 2012. 7(9): p. e44843
    [PMID:22984573]